*: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. # 3454A for complete product documentation and resources. *: Because the covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins, this ladder is not recommended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. Cat. This ladder is useful for monitoring of protein migration during SDS-PAGE and verifying transfer efficiency at western blotting. In this case, this ladder is sufficient for 50 lanes. 10 μl of this ladder is loaded per lane of SDS-PAGE minigel for standard use. This ladder is pre-mixed with loading buffer and ready to load. The 30 and 70 kDa bands have quadruple the mass of the other bands and serve as reference indicators. When this ladder is analyzed by SDS-PAGE, it is resolved into clearly identifiable visible bands from 10 to 210 kDa. CLEARLY Stained Protein Ladder is a mixture of 11 pre-stained recombinant highly purified proteins.
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